Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yuxin He
Date created: 2016-10-01 11:00:00
Date modified: 2016-10-19 01:59:53
dT+mhbR+mhbPt+GFP
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K2088002_sequence (Version 1) |
Description
PbaC is a 44-kDa protein that consists of 408 amino acids and contains a consensus motif for a flavin adenine dinucleotide-binding (ADP-binding) site (GXGX2GX3AX6G)。Existing in Sphingobium wenxiniae JZ-1, it???s a glutathione reductase (GR)-type reductase gene. Though pbaC is the important gene to degrade 3-PBA as the first step, the transcriptional level of pbaC was far less than that of other genes like pbaA1A2B since it is not located in the immediate vicinity of pbaA1A2B. However, with the optimization of our experiment, the efficience of degradation on 3-pba has been improved.
Notes
we have used diffrerent promotors and RBS to test the degrading efficience of pbaC. And we design the sequence as the part submission requests.Source
PbaC comes from Sphingobium wenxiniae JZ-1, and it exists on the Scaffold 12, instead of the vector of pbaA1A2B.Sphingobium wenxiniae JZ-1 utilizes a wide range of pyrethroids and their metabolic product, 3-phenoxybenzoate, as sources ofcarbon and energy.
GenBank: KJ009325.1
reference:
A Novel Angular Dioxygenase Gene Cluster Encoding 3-Phenoxybenzoate 1=,2=-Dioxygenase in Sphingobium wenxiniae JZ-1,
Chenghong Wang, Qing Chen, Rui Wang, Chao Shi, Xin Yan, Jian He, Qing Hong, Shunpeng Li
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
| Access | Instance | Definition |
| public public public | BBa_B0014 BBa_K2088011 BBa_K2088004 | BBa_B0014 BBa_K2088011 BBa_K2088004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0014 BBa_K2088011 BBa_K2088004 | 1,95 102,1019 1028,2348 | BBa_B0014 BBa_K2088011 BBa_K2088004 |