BBa_K2088009

BBa_K2088009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2088009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xia Chen

Date created: 2016-10-07 11:00:00

Date modified: 2016-10-18 04:16:07

nativeRBS-C23O with double Terminator

Details

• Types: DnaRegion
• Roles: Intermediate; engineered_region
• Sequences: BBa_K2088009_sequence (Version 1)

Description

This composite part includes a constitutive promoter (BBa_J23100), a catechol 2,3-dioxygenase gene with native rbs (BBa_K2088008) and the double terminator (BBa_B0015).
This device can be used as a reporter, because catechol 2,3-dioxygenase (C23O) can convert catechol to bright yellow product 2-hydroxymuconate semialdehyde.

Notes

Uses J23100, a strong Anderson Promoter, to produce high levels of expression of C23O.

Source

This is a compostie part including catechol 2,3-dioxygenase gene from Sphingobium sp. YBL2 encoded in part BBa_K2088008.

Components

Access	Instance	Definition
public	BBa_K2088008	BBa_K2088008
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2088008	1,1021	BBa_K2088008
BBa_B0015	1030,1158	BBa_B0015

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2088009/1

Member of these Collections

• iGEM Parts Registry