Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Edinburgh iGEM 2009
Date created: 2009-10-15 11:00:00
Date modified: 2015-05-08 01:11:30
PyeaR with Renilla luciferase
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K216014_sequence (Version 1) |
Description
This is nitrate- and nitrite-responsive promoter PyeaR of Escherichia coli joined to Renilla luciferase with the addition of a strong bacterial ribosome binding site. This should lead to production of light in the presence of nitrate/nitrate and coelenterazine.Notes
No special considerations. Sequencing suggests that there is an extra 'A' base following the scar between the RBS and the Renilla luciferase; that is, the scar region reads TACTAGAATG instead of TACTAGATG. If the sequencing is correct, this base must be present in J52008 or have been introduced by a random event during the construction process. This makes the gap between the RBS and start codon one base longer, but still well within the optimal range for E. coli and B. subtilis.Source
Made by joining PyeaR BioBrick BBa_K216005 to a PCR fusion of strong synthetic ribosome binding site J15001 and Renilla luciferase BioBrick J52008.| Access | Instance | Definition |
| public public public | BBa_K216005 BBa_J15001 BBa_J52008 | BBa_K216005 BBa_J15001 BBa_J52008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K216005 BBa_J15001 BBa_J52008 | 1,100 109,118 125,1060 | BBa_K216005 BBa_J15001 BBa_J52008 |