Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marissa Sumathipala
Date created: 2016-09-04 11:00:00
Date modified: 2016-09-15 01:44:50
Penicillium alpha amylase w/ no promoter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2185003_sequence (Version 1) |
Description
This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the fungus Penicilium that codes for an alpha amylase enzyme. This composite part contains the coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. It does not include a promoter. The amylase enzyme can be expressed by cloning this part into a plasmid with a promoter built in (such as the S.cerevisiae yeast vector pAG36).The components of this part are:
Kozak Sequence - BBa_K165002
This kozak sequence functions as the ribosome binding site for S.cerevisiae. It is involved in translating the sequence from mRNA to amino acids.
Mating Factor Secretion Tag - BBa_K792002
This part is the secretion signal peptide from the S.cerevisiae α-mating factor. This signal peptide directs the secretion of the produced protein, allowing the protein to interact with the environment outside the cell.
Fungal Alpha Amylase Coding Sequence - BBa_K2185002
This coding sequence codes for the alpha amylase enzyme from the Pencillium fungus. The enzyme can break alpha bonds linking polysaccharides, thus degrading starch into monosaccharides.
ADH1 Terminator - BBa_K392003
This is the terminator region from yeast alcohol dehydrogenase (ADH1) gene.
Notes
Removed Kpn and spe sites. Codon optimized for S.cerevisiae.Source
Coding sequence - pencilium fungus (sequence from GenBank)| Access | Instance | Definition |
| public public public public public public public | BBa_K165002 BBa_K792002 BBa_K165002 BBa_K792002 BBa_K392003 BBa_K2185002 BBa_K392003 | BBa_K165002 MFa1-Secre BBa_K165002 MFa1-Secre BBa_K392003 BBa_K2185002 BBa_K392003 |