BBa_K223051

BBa_K223051 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K223051

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anusuya Ramasubramanian

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:31

5MT operon (promoter-operator)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K223051_sequence (Version 1)

Description

This is a modified trp operon, promoter and operator site, that recognizes and binds to a doubly mutant repressor sequence and its corepressor, 5-methyl-L-tryptophan. In conjunction with the mutant tryptophan repressor, this modified operon functions as an inverter, converting input 5-methyl tryptophan levels and converting decreasing levels of 5MT into a PoPs output signal for downstream transcription.

Notes

Point mutation from A to C on the second aporepressor binding site (i.e. -18) on the sense strand.

Source

Originally taken from "Naturally occurring promoter down mutation: Nucleotide sequence of the trp promoter/operator/leader region
of Shigella dysenteriae 16" (Miozzari,G and Yanfosky, C. Proc. Natl. Acad. Sct. USA. Vol. 75, No. 11, pp. 5580-5584, November 1978. Genetics). Sequence modifications done by Stanford iGEM 2009.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K223051/1

Member of these Collections

• iGEM Parts Registry