Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:37
Strep-FluA-Middle Linker-Fok_i
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K243025_sequence (Version 1) |
Description
This combination of parts use the benefits of an Strep-tag and is linked with a FluroescineA tag. The MiddleLinker (GlySerGlyGly)x2 produce a certain distance between the FluA tag and the protein domain Fok_i.Notes
The linker length and the choice of the purification tag in combination with the anticalin tag allowed us several combination possibilities, which we had to prove to achieve the best combination.Source
Combined by serial clonig steps.| Access | Instance | Definition |
| public public public public public public public | BBa_K157012 BBa_B0105 BBa_K157004 BBa_B0105 BBa_K243005 BBa_B0105 BBa_K243001 | BBa_K157012 Scar 25 BBa_K157004 Scar 25 MiddleLink Scar 25 Fok_i |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K157012 BBa_B0105 BBa_K157004 BBa_B0105 BBa_K243005 BBa_B0105 BBa_K243001 | 1,24 25,30 31,552 553,558 559,582 583,588 589,1167 | BBa_K157012 Scar 25 BBa_K157004 Scar 25 MiddleLink Scar 25 Fok_i |