Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Romain Bodinier
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:42
fec promoter + RFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K257023_sequence (Version 1) |
Description
Promotor pFec , concerning the fec operon , is the one that will trigger a fluorescent response.Notes
we use the BBa_J61002 to have the pFec link to RFPSource
K12 genomic DNA| Sequence Annotation | Location | Component / Role(s) |
| BBa_J61002 RBS BBa_B0034 RBS mRFP BBa_B0015 rrnB terminator NotI PstI Verification reverse (VR) primer binding site ColE1 origin pBR322 origin beta-lactamase ampicillin resistance marker beta-lactamase (ampR, bla) promoter Verification forward (VF2) primer binding site BioBrick prefix Ambiguity; should be a T BBa_K257000 -35 -10 +1 important for activity | 1,2934 1,12 1,12 19,699 733,861 744,787 870,877 877,882 940,959 1041,1723 1044,1663 1818,2678 2720,2748 2801,2820 2919,2940 2933,2933 2941,3044 2976,2981 2997,3002 3011,3011 3011,3030 | engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/BioBrick engineered_region feature/cds CDS feature/BioBrick engineered_region feature/stem_loop stem_loop feature/dna sequence_feature feature/dna sequence_feature feature/primer_binding primer_binding_site feature/dna sequence_feature sequence_feature feature/dna feature/cds CDS feature/promoter promoter feature/primer_binding primer_binding_site sequence_feature feature/dna feature/mutation sequence_alteration engineered_region feature/BioBrick feature/operator operator operator feature/operator sequence_feature feature/misc feature/misc sequence_feature |