Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
Date created: 2009-10-09 11:00:00
Date modified: 2015-05-08 01:11:42
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K258008_sequence (Version 1) |
Description
EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37??C, the optimum growth temperature for E. coliNotes
We used whole plasmid in order to excrete a fusion protein with one of two signal tags which are TliA and LARD1Source
Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor| Access | Instance | Definition |
| public public public public public public public public public public public | BBa_R0040 BBa_B0034 BBa_K258100 BBa_B0010 BBa_B0034 BBa_K258101 BBa_B0010 BBa_B0034 BBa_K258102 BBa_B0010 BBa_B0012 | p(tetR) BBa_B0034 BBa_K258100 BBa_B0010 BBa_B0034 BBa_K258101 BBa_B0010 BBa_B0034 BBa_K258102 BBa_B0010 BBa_B0012 |