Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Petros Mina
Date created: 2009-10-14 11:00:00
Date modified: 2015-05-08 01:11:42
AraC-RBS-OsmE
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K259012_sequence (Version 1) |
Description
This part will express the protein OsmE that is found on the Outer Membrane of E.coli cells. This protein is osmotically inducible but of unknown function.The part currently carries an illegal EcoRI site that can be mutated out (contact us for information).
It is useful for protein fusions when the user wants to deliver proteins to the outer membrane. For protein fusions the part can be used with Bioscaffolds designed by the BCCS-Bristol iGEM'09 team, that facilitate protein fusion (
At the time of writing only part Bba_K259002 is supported.
Notes
Part was designed using 2-way ligation to avoid EcoRI site.Source
AraC - occurs naturally in E.coli genome. This part was created by assembling parts R0081 and R0080 together.RBS - Designed from the Anderson family. A strong RBS for high translation rates.
OsmE - Obtained via PCR gene cloning from the E.coli MG1655 genome.
| Access | Instance | Definition |
| public public public public | BBa_R0081 BBa_R0080 BBa_J61100 BBa_K259011 | AraC O2 AraC BBa_J61100 BBa_K259011 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0081 BBa_R0080 BBa_J61100 BBa_K259011 | 1,183 192,340 349,360 367,708 | AraC O2 AraC BBa_J61100 BBa_K259011 |