Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-16 11:00:00
Date modified: 2015-05-08 01:11:42
pTetFlp. [anhydrotetracycline] -> FLP(F70L)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K260002_sequence (Version 1) |
Description
This plasmid is based on pSC101 and contains TetR as well as the F70L variant of FLP recombinase under tet-promoter control. Therefore anhydrotetracycline can be used to induce FLP(F70L) expression.The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.
Notes
dhfr had to be deleted as the same gene for trimpethoprim resistance was going to be used elsewhere in the same cell.Source
This plasmid comes fromSarov M, Schneider S, Pozniakovski A, Roguev A, Ernst S, Zhang Y, Hyman AA, Stewart AF. A recombineering pipeline for functional genomics applied to Caenorhabditis elegans. Nat Methods. 2006 Oct;3(10):839-44.
However, it was modified by deletion of dhfr gene and RedABGRecA operon through insertion of a kanamycin resistance cassette.
| Sequence Annotation | Location | Component / Role(s) |
| suffix pSC101 origin repA TetR HygR KanR FLP(F70L) T4_32 terminator tet-promoter prefix | 1,21 75,436 820,1770 1812,2435 2445,3470 4144,4938 5245,6516 5193,5224 6517,6643 6680,6701 | feature/BioBrick engineered_region feature/misc sequence_feature CDS feature/cds feature/cds CDS CDS feature/cds CDS feature/cds feature/cds CDS stem_loop feature/stem_loop feature/promoter promoter feature/BioBrick engineered_region |