Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:42
pMA-RQ-@CC2FosMB-10000bp
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K260008_sequence (Version 1) |
Description
This plasmid backbone is based on pMA-RQ from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 10000 locus of pCC2FosMB ([[Part:BBa_K260001|]]) by recombineering.The pMA-RQ-@CC2FosMB-10000bp backbone is available with [[Part:BBa_K260015|]], which is composed of promoter-FRT-dhfr. If the PvuII fragment from [[Part:BBa_K260008|]]-[[Part:BBa_K260015|]] is transferred by recombineering to the the 10000 locus of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]], the intervening sequence between the FRT sites of [[Part:BBa_K260015|]] and [[Part:BBa_K260016|]] on the [[Part:BBa_K260001|]] backbone will be 10,000 bp exactly.
These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically [[Part:BBa_K260015|]]) to the pCC2FosMB backbone [[Part:BBa_K2600001|]]. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above:
[[Part:BBa_K260004|]]: pMA-CC2FosMB-00500bp
[[Part:BBa_K260005|]]: pMA-CC2FosMB-01000bp
[[Part:BBa_K260006|]]: pMA-CC2FosMB-02000bp
[[Part:BBa_K260007|]]: pMA-CC2FosMB-05000bp
[[Part:BBa_K260008|]]: pMA-RQ-CC2FosMB-10000bp
Notes
The 50 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]] and put the FRT site of [[Part:BBa_K260015|]] (if used with the present backbone [[Part:BBa_K260008|]]) at a defined distance from the FRT site of [[Part:BBa_K260016|]].Source
Geneart plasmid pMA-RQ, with 2x 50 bp from pCC2FosMB [[Part:K260001|]].| Sequence Annotation | Location | Component / Role(s) |
| suffix BBa_K260001 (pCC2FosMB) 3' homology FRT-10000bp PvuII site PvuII site AmpR ColE1 origin BBa_K260001 (pCC2FosMB) 5' homology FRT-10000bp prefix | 1,21 22,91 92,97 2451,2456 1087,1947 272,939 2457,2526 2527,2548 | engineered_region feature/BioBrick sequence_feature feature/misc sequence_feature feature/dna sequence_feature feature/dna feature/cds CDS feature/misc sequence_feature sequence_feature feature/misc engineered_region feature/BioBrick |