Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Richard Hauser
Date created: 2009-10-14 11:00:00
Date modified: 2015-05-08 01:11:43
OmpA with proteolytic cleavage tag
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K265011_sequence (Version 1) |
Description
This part is a fusion of a modified OmpA with a signal sequence to allow cleavage when a protein is fused. OmpA was modified by replacing the normal restriction sites with Tom Knight's fusion format.An OmpA/protein fusion normally allows display of proteins on the cell surface. The addition of this signal sequence allows secretion by allowing cleavage after display.
Notes
NoneSource
The OmpA was part BBa_K103006 with a couple modified restriction sites. The signal sequence was synthesized by Mr. Gene.| Sequence Annotation | Location | Component / Role(s) |
| NdeI BBa_K103006 ATG OmpA linker BBa_K265002 Proteolytic Cleavage Sequence | 1,7 1,464 4,6 4,438 439,464 471,584 471,584 | feature/misc sequence_feature feature/BioBrick engineered_region feature/start start_codon feature/cds CDS feature/cds CDS feature/BioBrick engineered_region feature/cds CDS |