BBa_K316022

BBa_K316022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K316022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-22 11:00:00
Date modified: 2015-05-08 01:11:56

B.subtilis transformation vector, targets amyE locus



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K316022_sequence (Version 1)

    Description

    This vector has been designed using the amyE 5' and 3' integration sequences for integration into B.subtilis genome

    AmyE locus
    This vector has been designed using the amyE 5' BBa_K143008 and 3 BBa_K143009' integration sequences for integration into B. subtilis genome. Insertion into the amyE locus provides a selection marker as the bacterium will no longer be able to breakdown starch. An iodine assay can be used to confirm integration. This phenotype makes the transformed bacterium considerably less likely to survive in natural environments.

    Chloramphenicol Resistance
    This vector also contains a positive selection marker, flanked by two dif sites. Chloramphenicol acetyltransferase BBa_J31005 provides resistance to chloramphenicol antibiotic. Dif BBa_K316002 sites allow excision of the antibiotic marker after integration, thus allowing the same marker to be used again or as a precaution against horizontal gene transfer.

    Blunt end cloning site
    PmeI restriction site BBa_K316013 is designed as a cloning site. Due to the 8bp recognition sequence it is a rare site that can be used to cut the vector only once.


    Please see ???Part Design??? section for design considerations and parts used.

    Source

    Existing biobricks, BBa_K143070, BBa_K316002, BBa_K316014 BBa_K143002

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K316022/1
     

    Attachments

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