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BBa_K316027

BBa_K316027 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K316027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-25 11:00:00

Date modified: 2015-05-08 01:11:56

B.subtilis transformation vector with LacI

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K316027_sequence (Version 1)

Description

yey

Notes

This part is designed for integration into B. subtilis' genome at amyE locus, it contains Spectinomycin resistance selection marker and constitutively expresses LacI. PmeI can be used to ligate a gene of interest using blunt ended methods.

Source

Existing biobricks, BBa_K143070, BBa_K316002, BBa_K316014 BBa_K143002

Components

Access	Instance	Definition
public public public public public public public	BBa_K143001 BBa_K143053 BBa_K143065 BBa_K143053 BBa_K143062 BBa_K316014 BBa_K143002	amyE 5 IS Pveg-spoVG Aad9-T Pveg-spoVG LacI-T BBa_K316014 amyE 3 IS

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143001 BBa_K143053 BBa_K143065 BBa_K143053 BBa_K143062 BBa_K316014 BBa_K143002	1,522 531,647 654,1561 1570,1686 1693,2915 2924,2967 2976,3977	amyE 5 IS Pveg-spoVG Aad9-T Pveg-spoVG LacI-T BBa_K316014 amyE 3 IS

Other Properties

igem#experience	Works
igem#partStatus	Released HQ 2013
igem#sampleStatus	In stock
igem#status	Available
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K316027/1