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BBa_K318026

BBa_K318026 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K318026

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nathaniel Pantalone, Justin Vrana

Date created: 2010-07-12 11:00:00

Date modified: 2015-05-08 01:11:57

pCons + attB + RBS + GFP + T

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Details

Types	DnaRegion
Roles	engineered_region Generator
Sequences	BBa_K318026_sequence (Version 1)

Description

This composite part is a GFP generator plus a constitutive promoter with a P22 attB recombination site. Using the P22 Int and a vector with the attP recombination site, it is possible to insert DNA upstream of the GFP generator. DNA insertion or recombination via P22 Int should end constitutive expression of the GFP. It is composed of the following biobricks: BBa_J23100, BBa_I11032, and BBa_E0240.

Notes

Slightly less expression b/c of distance between promoter and gene.

Source

biobricks

Components

Access	Instance	Definition
public public public public public public	BBa_J23100 BBa_I11032 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012	BBa_J23100 P22 attB BBa_B0032 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23100 BBa_I11032 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012	1,35 44,70 79,91 98,817 826,905 914,954	BBa_J23100 P22 attB BBa_B0032 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K318026/1