Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Justin Vrana, Nate Pantalone
Date created: 2010-07-12 11:00:00
Date modified: 2015-05-08 01:11:58
lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K318030_sequence (Version 1) |
Description
This composite part is a prefix ???lock??? cassette requiring consecutive recombination by Hin and Cre, in that order, to express a gene downstream of the hixC site. The SapI restriction site can be used to insert DNA with ATC sticky ends in order to modify the length of recombination. After Hin recombination, the part becomes: lox66 + rpCons + hixC + HindIII + rHin enhancer + T + T + SapI + lox71 + hixC. When Cre is used after Hin, the part becomes: mloxP + SapI + T + T + Hin enhancer + HindIII + hixC + pCons + loxP + hixC.Notes
Note that the constitutive promoter is oriented to express whatever gene is downstream of the final hixC site.Source
From Synthesis| Access | Instance | Definition |
| public public public public public public public public public | BBa_I718016 BBa_K318050 BBa_J44000 BBa_I718017 BBa_K318051 BBa_B1006 BBa_B1006 BBa_K318052 BBa_J44000 | lox66 BBa_K318050 hixC lox71 BBa_K318051 BBa_B1006 BBa_B1006 BBa_K318052 hixC |