Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anish Kapadia
Date created: 2010-06-22 11:00:00
Date modified: 2015-05-08 01:12:11
catechol 1 2-dioxygenase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K357000_sequence (Version 1) |
Description
Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. This class of enzymes incorporate dioxygen into the substrate. There are several family members in the Catechol dioxygenases family with different specificities, these include; catechol 1,2-dioxygenase (EC 1.13.11.1), catechol 2,3-dioxygenase (EC 1.13.11.2), and protocatechuate 3,4-dioxygenase (EC 1.13.11.3). The active site of catechol dioxygenases most frequently contains iron, but manganese-containing forms are also known. Catechol 1 2-dioxygenase utilizes Iron at its active site and it converts catechol into cis,cis-muconic acid.Notes
The sequence is was changed from the original to remove a PstI restriction site located around bp 263, to remove the restriction site while maintaining a conserved aa sequence.Source
This part is designed from genomic sequence:AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D.,
Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,
Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,
Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,
Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,
Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,
Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,
Duesterhoft,A., Tummler,B. and Fraser,C.
TITLE Complete genome sequence and comparative analysis of the
metabolically versatile Pseudomonas putida KT2440
JOURNAL Environ. Microbiol. 4 (12), 799-808 (2002)
| Sequence Annotation | Location | Component / Role(s) |
| Start Codon Removed EcoR1 Restriction Site Stop Codon | 1,3 514,519 934,936 | feature/start start_codon feature/mutation sequence_alteration feature/stop stop_codon |