Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: syed imtiyaz
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:17
AHL induced concentration Band detect
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K379002_sequence (Version 1) |
Description
This component detects the concentration of AHL and activates the reporter protein (GFP/RFP/YFP) when the concentration is in the correct band. If the concentration of AHL is either higher or lower than the band specific to this component, the reporter is not activated.Notes
We had to look for different types of LacI degradation tags to obtain the correct configuration.Source
lux pLR0063
.
B0034
luxr
C0062
.
B0010
.
B0012
lux pR
R0062
.
I746352
.
B0010
.
B0012
.
I746364
.
B0034
cI lam
C0051
.
B0010
.
B0012
cI lam
R0051
.
B0034
lacI
C0012
.
B0010
.
B0012
.
I746365
.
B0034
lacI
C0012
.
B0010
.
B0012
LacI
R0010
.
B0034
YFP
E0430
.
B0010
.
B0012
| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public public public public public public public public public public public public public | BBa_R0063 BBa_B0034 BBa_C0062 BBa_B0010 BBa_B0012 BBa_R0062 BBa_I746352 BBa_B0010 BBa_B0012 BBa_I746364 BBa_B0034 BBa_C0051 BBa_B0010 BBa_B0012 BBa_R0051 BBa_B0034 BBa_C0012 BBa_B0010 BBa_B0012 BBa_I746365 BBa_B0034 BBa_C0012 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0034 BBa_E0430 BBa_B0010 BBa_B0012 | lux pL BBa_B0034 luxr BBa_B0010 BBa_B0012 lux pR BBa_I746352 BBa_B0010 BBa_B0012 BBa_I746364 BBa_B0034 cI lam BBa_B0010 BBa_B0012 cI lam BBa_B0034 lacI BBa_B0010 BBa_B0012 BBa_I746365 BBa_B0034 lacI BBa_B0010 BBa_B0012 LacI BBa_B0034 BBa_E0430 BBa_B0010 BBa_B0012 |