Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cody Tramp
Date created: 2010-07-31 11:00:00
Date modified: 2015-05-08 01:12:19
art+ secretion tag for Synechocystis sp. PCC 6803
| Types | DnaRegion |
| Roles | Tag tag |
| Sequences | BBa_K390004_sequence (Version 1) |
Description
In Synechocystis sp. PCC 6803, the system of secretion is based on the charge of the N-terminus leader sequence. Positive charges on the N-terminus leader sequence contribute to secretion in a non-sequence-dependent manner (Sergeyenko TV and Los DA. 2003. Cyanobacterial leader peptides for protein secretion. FEMS Microbiology Letters 218: 351-357).This part is an artificially designed secretion tag from the above paper. It is composed of positively charged amino acids, and was shown to successfully function as a secretion tag in Synechocystis in the above paper.
Notes
noneSource
This part is based on a sequence that was artificially designed in Sergeyenko and Los, 2003. It was created by annealing two oligionucleotides together.| Sequence Annotation | Location | Component / Role(s) |
| Start Codon secretion tag - Synechocystis sp. PCC 6803 | 1,3 1,64 | start_codon feature/start feature/tag tag |