BBa_K416000

BBa_K416000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K416000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Russell Durrett

Date created: 2010-05-27 11:00:00

Date modified: 2015-05-08 01:12:27

Aga2 CDS Responsible for Yeast Surface Display

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_K416000_sequence (Version 1)

Description

The Aga2 protein is fused in frame with a protein that the user wants to display on the yeast cellular surface. Yeast cells constitutively expressing Aga1 (strain EBY100) secrete the Aga2 fusion protein, which associates with Aga1 through two disulfide bonds and quaternary structure to anchor Aga2 to the surface. The fused protein then is displayed on the surface of the yeast cell.

The STOP codon at the end of the CDS was removed to allow for N- and C-terminus fusions.

Notes

We removed the STOP codon to allow for N- and C-terminal fusions, which have both been proven to occur efficiently.

Source

This parts was biobricked from the pCTCON2 plasmid which was sent to us by Dr. D. K. Wittrup at MIT.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Aga2 CDS	1,261	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K416000/1

Member of these Collections

• //chassis/eukaryote/yeast
• //proteindomain/localization
• iGEM Parts Registry