Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jan Marte Contreras Ortiz
Date created: 2010-10-21 11:00:00
Date modified: 2015-05-08 01:12:29
Intelligent arabinose biosensor with differential response
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K427009_sequence (Version 1) |
Description
This part was the final construct of the Tec-Monterrey iGEM Team in 2010. It senses the concentration of arabinose through the PBad family of promoters. Then amplifies it through sensitivity tuners, the activator proteins of the tuners are jammed by reverse arabinose promoters. The sensitivity tuners activate the production of the three fluorescent proteins. This device was created as a proof of concept for the combination of the jammers and sensitivity tuners, rather than using the whole part, the construct design is what should be regarded.Notes
There were no special considerations. This sequence is a proof of concept of the combination of the jammer and sensitivity tuner devices.Source
The parts used to build this device came from several sources. The proteins C and Mor, as well as the Pm and Pmom promoters come from slight modifications of the reported genome of the Mu bacteriophage. The pBad family of promoters comes from British Columbia's 2009 project and are modifications of the wt PBad promoter from E. coli. The other activator and promoter come from Cambridge's 2007 project while the fluorescent proteins and terminators come from the registry of parts.| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public public | BBa_B0014 BBa_K206000 BBa_K427002 BBa_J44002 BBa_B0014 BBa_I13453 BBa_I746352 BBa_K427000 BBa_B0014 BBa_K206001 BBa_K427001 BBa_B0014 BBa_K427003 BBa_E0240 BBa_I746361 BBa_E0430 BBa_K427004 BBa_J06702 | BBa_B0014 pBAD BBa_K427002 BBa_J44002 BBa_B0014 BBa_I13453 BBa_I746352 pBad Weak BBa_B0014 BBa_K206001 BBa_K427001 BBa_B0014 BBa_K427003 GFP report BBa_I746361 BBa_E0430 BBa_K427004 mCherry |