Types | DnaRegion
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Roles | Coding
CDS
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Sequences | BBa_K511203_sequence (Version 1)
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Description
This part encodes a transactivator composed of the DNA-binding domain for the tetracycline operator sequence and the viral VP16 transactivation domain; this protein has been engineered over previous versions to be more sensitive to tetracycline and its analogues.
Figures 1 and 2 below show expression from a promoter with six tetracycline response elements (TRE-Tight) in the presence of rtTA3 and various levels of doxycycline, a tetracycline analogue.
Notes
Not applicable.
Source
This protein is a heavily engineered fusion between the tetracycline operator binding domain from TetR and the viral VP16 transactivation domain from the herpes simplex virus.