BBa_K518004

BBa_K518004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K518004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Masato Ohgishi

Date created: 2011-09-14 11:00:00

Date modified: 2015-05-08 01:12:33

IPTG-inducible L-Asp producing device

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K518004_sequence (Version 1)

Description

AspA is an aspartate ammonia-lyase, which produces aspartate from fumarate and ammonium ion. Aspartate can be used as a chemoattractant for bacteria including Escherichia coli.
In this device, AspA CDS, flanked with RBS and terminator, is under control of lacI-regulated strong promoter. IPTG can be used to induce aspartate production.

Notes

None.

Source

From BioBrick parts.

Components

Access	Instance	Definition
public	BBa_R0011	lacI+pL
public	BBa_B0032	BBa_B0032
public	BBa_C0083	aspA
public	BBa_B0014	BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011	1,54	lacI+pL
BBa_B0032	64,76	BBa_B0032
BBa_C0083	83,1625	aspA
BBa_B0014	1634,1728	BBa_B0014

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K518004/1

Member of these Collections

• //function/motility
• iGEM Parts Registry