BBa_K561004

BBa_K561004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K561004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: LI Ye, XIA Yu
Date created: 2011-10-02 11:00:00
Date modified: 2015-05-08 01:12:41

PfdhF+cex+cenA+tetR



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K561004_sequence (Version 1)

Description

Bottom Layer of the Rainbo-film expression system for cellulose degradation. Exoglucanase (Cex) and endoglucanase A (CenA) form Cellulomonas fimi (iGEM08_Edinburgh) are under the regulation of anaerobic promoter fdhF. The expression of tetR will repress Ptet, which in the device of upper layer expression in the biofilm.

Notes

This device has strong RBS.
Cex and CenA are expressed in the same time for the efficient degradation of cellulose into cellobiose.

Source

PfdhF comes from E.coli genome.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K561004/1