BBa_K607019

BBa_K607019 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K607019
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Olesja Popow
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:52

Prm_cI_antiPlasB with 0.07RBS



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K607019_sequence (Version 1)

Description

cI without degradation tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))

Notes

We decided to keep PAI concentrations in the media constant (by adding N-(3-oxododecanoyl)homoserine lactone) and control the expression of LasR protein in order to achieve tight control of the reverse antisense lasB promoter.

Source

2011 distribution

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BBa_K607019/1