BBa_K607027

BBa_K607027 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K607027
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Olesja Popow
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:52

PlasB_LasR-DAS_antipBad/araC with 0.07RBS



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K607027_sequence (Version 1)

Description

LasR protein with moderately fast degradation tag under transcriptional control of PlasB promoter and reverse antisense PBad promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.

Notes

none

Source

2011 distribution

igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K607027/1