BBa_K648012

BBa_K648012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K648012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jim Rose
Date created: 2011-07-03 11:00:00
Date modified: 2015-05-08 01:12:59

LacZa wtih Standard 25 Prefix/Suffix



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K648012_sequence (Version 1)

    Description

    This is the lacZa subunit used to complement cells for blue/white screening. It has been cloned into a vector including the prefix/suffix required for standard 25 assembly for fusion proteins.

    Notes

    This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly.

    It was synthesized through PCR oligo synthesis methods using the following primers:

    Forward
    TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCACCATGATTACGGATTCAC (57.74 oC)

    Reverse
    ATAACTGCAGCGGCCGCTACTAGTATTAACCGGTTCACTCCAGCCAGC (55.05 oC)

    Source

    This part was amplified out of registry stock of LacZa in order to include the standard 25 prefix/suffix

    Sequence Annotation Location Component / Role(s)
    protein
    		1,228
    		feature/protein CDS

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K648012/1
     

    Attachments

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