Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kenta Mine
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:13
P3HB + eYFP producing construct
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K759013_sequence (Version 1) |
Description
This is an construct for producing P3HB and eYFP (enhanced yellow fluorescent protein). A constitutive promoter(BBa_R0040) locates on the upstream of the whole construct and by adding 2 percent gulcose into the media, the bacteria would produce bioplastics and eYFP. eYFP exists on the downstream of P3HB production operon for estimating a relationship between amount of produced bioplastics and expressed bioplastic producing enzymes .Notes
phaA and phaB was cloned from pGEM phbCAB which carries the gene of Ralstonia Eutropha.Source
Nothing to remark.| Access | Instance | Definition |
| public public public | BBa_R0040 BBa_K759009 BBa_E0430 | p(tetR) BBa_K759009 BBa_E0430 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_K759009 BBa_E0430 | 1,54 63,3843 3852,4729 | p(tetR) BBa_K759009 BBa_E0430 |