BBa_K766000

BBa_K766000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K766000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yu Zhao

Date created: 2012-09-21 11:00:00

Date modified: 2015-05-08 01:13:14

AHL synthetase LuxI with His-tag

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K766000_sequence (Version 1)

Description

This part consists of LuxI synthase with His-tag at the N-terminal of the protein. With the help of His-tag, you can detect the expression of this enzyme. Besides, you can purify the protein using Ni-colume and gel filtraion. As a result, you can build a system to product C6HSL in vitro. Between the His-tag and protein, we add the cutting site of thrombin. So that you can cut off the his-tag from the protein.

Notes

We want to find a way to detect the expression and purify LuxI protein.

Source

This part is from BBa_C0261. We make a truncation on the C-terminal to cut off the LVA tag. His tag and thrombin cutting site is add on the N-terminal.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Poly-hig tag	13,30	tag, feature/tag
LuxI without LVA tag	70,639	feature/cds, CDS
thrombin cutting site	40,57	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K766000/1

Member of these Collections

• iGEM Parts Registry