Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Chen
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:18
[R0010][B0034] - mgfp-5-FliC Insertion Chimera
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K781002_sequence (Version 1) |
Description
This is an mgfp-5 chimeric insertion flagella.Notes
The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera.Source
This was created using PCR Overlap extension from K781000| Sequence Annotation | Location | Component / Role(s) |
| end of LacI coding region (inactive) BBa_R0010 CAP binding site -35 -10 LacI binding site start BBa_B0034 conserved Flagellin Linker 1 K197018 Linker 2 Flagellin | 1,88 1,200 89,126 137,142 161,166 166,200 173,173 209,220 213,216 230,976 977,997 998,1225 1226,1245 1246,1996 | CDS feature/cds engineered_region feature/BioBrick feature/binding non_covalent_binding_site feature/promoter promoter promoter feature/promoter non_covalent_binding_site feature/binding start_codon feature/start engineered_region feature/BioBrick feature/conserved conserved_region feature/protein CDS feature/protein CDS engineered_region feature/BioBrick feature/protein CDS feature/protein CDS |