BBa_K784053

BBa_K784053 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K784053
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Asaf Blumental
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:22

theophylline riboswitch + T7*(T3) RNAP



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K784053_sequence (Version 1)

Description

This part contains an engineered theophylline riboswitch (RS) (BBa K784005) and an engineered T7*(T3) RNA polymerase (K784052). The riboswitch contains a RBS and it will be exposed and starts translating the T7*(K1F) RNAP only after inserting of theophylline.

Notes

The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7*(T3) RNAP are adjacent to one another without any restriction site between them.

Source

Assembly PCR of BBa K784005 and K784052

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K784053/1