BBa_K797011

BBa_K797011 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K797011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tomohiro Nobeyama

Date created: 2012-09-20 11:00:00

Date modified: 2015-05-08 01:13:23

Plasmid include T7 promoter, Histag and R9 peptide sequence

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K797011_sequence (Version 1)

Description

This plasmid has modified T7 promoter,RBS,His tag and nine Argenine signal peptide(R9 peptide). R9 peptide cause endocytosis of mammalian and plant cells and make them take not only R9 peptide but also Proteins combined to this peptide. you can correct and purify products of this promoter by using anti-His tag antibody.

Source

This part is synthesized artificially

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
prefix	1,22	feature/misc, sequence_feature
T7	23,42	feature/promoter, promoter
RBS	54,65	ribosome_entry_site, feature/rbs
6His	75,92	feature/tag, tag
R9	93,119	feature/tag, tag
suffix	121,141	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K797011/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors