BBa_K804007

BBa_K804007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K804007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joe Ho
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:25

Constitutive ECFP generator - Arabinose inducible TrpA coding gene



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K804007_sequence (Version 1)

Description

This part contains a constitutive ECFP generator along with an arabinose inducible (Pbad) Trp A coding gene. Upon induction with arabinose, TrpA is expressed for indole-3-glycerol-phosphate(InGP)binding and catalysis/cleavage of InGP to indole and glyceraldehyde-3-phosphate during an α reaction. This construct can be used to induce growth in Trp- auxotrophs and can be monitored by fluorescence for its population dynamics in co-culture with other auxotrophs.

Notes

None available.

Source

Strong Constitutive Promoter-ECFP generator- BBa_K804000
Pbad inducible Promoter- BBa_I13453
RBS-BBa_B0034
TrpA coding gene- BBa_K804002
Terminator- BBa_B0025

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K804007/1