Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cyrille Pauthenier
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:27
RBS B0034 with GoldenBrick sequence
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_K812053_sequence (Version 1) |
Description
This part is the GoldenBricked version of B0034 RBS. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed.To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB
Notes
This part has been amplified from B0034 with GoldenBrick extension by PCR and cloned in pSB1C3 using EcoRI and PstISource
This part has been amplified from B0034 with GoldenBrick extension| Sequence Annotation | Location | Component / Role(s) |
| GoldenBrick RBS Prefix GoldenBricked RBS B0034 Goldenbrick RBS Sufix EcoRI BsalI XbaI SpeI BsaI PstI OH2 Spacer OH3 | 1,17 18,29 30,47 1,6 7,12 11,16 31,36 37,42 43,47 14,17 30,30 32,35 | engineered_region feature/BioBrick ribosome_entry_site feature/rbs engineered_region feature/BioBrick feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |