Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Steven Allen
Date created: 2012-09-30 11:00:00
Date modified: 2015-07-24 01:19:31
Cadmium Promoter with GFP Reporter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K824008_sequence (Version 1) |
Description
We digested the promoter responsive to Cadmium (BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium and Arsenic, respectively.Notes
noneSource
This part is a combination of 2007 Brown's iGEM part BBa_K174015 and MIT's part (from 2005) I13401.| Access | Instance | Definition |
| public public | BBa_K174015 BBa_I13401 | BBa_K174015 BBa_I13401 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K174015 BBa_I13401 | 1,205 212,1068 | BBa_K174015 BBa_I13401 |