Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kuanwei Sheng
Date created: 2012-09-07 11:00:00
Date modified: 2015-05-08 01:13:35
PfadR, synthetic promoter with tandem FadR binding site
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K861060_sequence (Version 1) |
Description
At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is place downstream of promoter J23110 with 3 base pair overlap.We test its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1.
Notes
NoneSource
Escherichia coli str. K-12 substr. MG1655| Sequence Annotation | Location | Component / Role(s) |
| FadR tandem binding site | 33,76 | non_covalent_binding_site feature/binding |