BBa_K912036

BBa_K912036 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K912036
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yuan Tao
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:45

pH Inducible GFP system (m)



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K912036_sequence (Version 1)

Description

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector. This particular part was constructed in order to test the to make sure that the Pgad/clc system works correctly. We hoped that it would be easier to see GFP for assay purposes. The original device is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.

The chloride antiporter was promoted by a strong promoter.

Notes

none

Source

GFP:registry CP/RBS: registry clc-ec1: PCR from genome Pgad: PCR from vector from CUHK 2011 team

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K912036/1