BBa_K942001

BBa_K942001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K942001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Luis Mario Leal Garza

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:48

scFv anti-His

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K942001_sequence (Version 1)

Description

This part is a single chain variable fragment (scFv), this is a fusion of the variable domains of antibodies. The antigen this scFv binds to with a greater stability is a C-terminal 6xHis tag; nevertheless it binds to tags with as less as 3xHis. It is formed by two domains, the variable heavy chain domain (VH) and the variable light chain domain (VL).

About safety
The part itself does not present any biohazard characteristic, but it is important to remember that it is designed to work in Pichia pastoris and that a biosecurity chamber is recommended to handle this organism,

Notes

This part is codon-optimized to be expressed in yeast. It is composed by two domains fused by a (GGGS)4 linker.

Source

As a fusion protein, the DNA of this scFv was synthetized artificially, its parts comes from a murine antibody.

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K942001/1

Member of these Collections

• iGEM Parts Registry