BBa_M10034

BBa_M10034 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M10034
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sadao Ota
Date created: 2009-05-09 11:00:00
Date modified: 2015-05-08 01:13:51

{˂
Ag43_short!}, for Ag 43 autotransporter display system, only transporter



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_M10034_sequence (Version 1)

    Description

    This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at
    This part is an actual transporter portion of an antigen 43 autotransporter display system based on this reference: PMID: 12107137.

    Notes

    Bglbricking of M10034, Ag43 (autotransporter only)
    PCR Oso001 and Oso002 on E. coli MG1655 genomic DNA (937 bp, EcoRI/BamHI)
    Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, L)
    Product is pBca9495KC-M10034 {

    Oso001 Cloning of Ag43, short ccataGAATTCatgAGATCTggtgaaaacaacagcgtccg
    Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg

    Source

    The source of this part can be found from Escherichia coli str. K-12 substr. MG1655, complete genome.

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M10034/1
     

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