BBa_M45117

BBa_M45117 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M45117

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Federico C. Rodriguez

Date created: 2014-04-18 11:00:00

Date modified: 2015-05-08 01:14:08

Uranium Inducible GFPuv Reporter

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_M45117_sequence (Version 1)

Description

This composite part is made out of the uranium inducible (urcA)promoter (BBa_M45114), a ribosome binding site, the GFPuv protein which is a green fluorescent protein (GFP) variant optimized for excitation by UV light, and an extra stop codon to prevent RNA polymerase from reading it through.

Notes

The complete sequence complied with RFC-10 assembly compatibility as a requirement for Utah State University Synthetic Biology class, so no design considerations had to be taken into account during the creation of the sequence as a biobrick.

Source

This composite part comes from BBa_M45113 + BBa_B0034 + BBa_M45116 + BBa_B0010 + BBa_B0012.

Components

Access	Instance	Definition
public	BBa_M45113	BBa_M45113
public	BBa_B0034	BBa_B0034
public	BBa_M45116	BBa_M45116
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_M45113	1,974	BBa_M45113
BBa_B0034	983,994	BBa_B0034
BBa_M45116	1001,1717	BBa_M45116
BBa_B0010	1726,1805	BBa_B0010
BBa_B0012	1814,1854	BBa_B0012

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M45117/1

Member of these Collections

• iGEM Parts Registry