BBa_M45504

BBa_M45504 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M45504
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dallin V. Christensen
Date created: 2015-04-22 11:00:00
Date modified: 2015-05-08 01:14:09

PF promoter with cell lysis device



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_M45504_sequence (Version 1)

Description

This is the cell lysis device designed by UC Berkeley in 2008 with the PF promoter that is activated by the protein phiR73. The lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin. Antiholin has its own constitutive promoter to prevent the formation of holin multimers from basal expression. When the device is off, higher expression of antiholin compared to holin is required for better stability of the device.

Notes

none

Source

Enterobacteria phage T4

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M45504/1