Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Heather Keller
Date created: 2007-03-05 12:00:00
Date modified: 2015-05-08 01:14:16
R0011+B0100+B0048
| Types | DnaRegion |
| Roles | engineered_region Intermediate |
| Sequences | BBa_S00160_sequence (Version 1) |
Description
This intermediate part is a composite of parts R0011 (lambda cI promoter with lacI binding sites) + B0100 (OmpA 5' UTR region containing two stabilizing hairpins) + B0048 (AvrII restriction site), formed via blunt cloning (does not contain biobricks mixed sites).Notes
This part was assembled via PCR amplification rather than with standard assembly in order to reduce cloning time (less assembly steps) and for ease of handling (small parts are hard to digest and purify properly).Source
This part was made by PCR amplification of B0100 from the MG1655 genome with the Biobricks upstream cloning sites (EcoRI and XbaI) and R0011 included in the forward promoter and B0048 and the Biobricks downstream cloning sites (SpeI and PstI) included in the reverse primer.| Sequence Annotation | Location | Component / Role(s) |
| R0011 R0048 R0100 lacO1 -35 lacO1 -10 hp1 hp2 ss2 AvrII site ss1 | 1,55 171,176 56,170 3,19 20,25 26,42 43,48 56,118 130,158 159,170 171,176 119,129 | feature/BioBrick engineered_region engineered_region feature/BioBrick engineered_region feature/BioBrick operator feature/operator feature/promoter promoter operator feature/operator feature/promoter promoter feature/stem_loop stem_loop feature/stem_loop stem_loop sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |