BBa_K2110809
BBa_K2110809 Version 1 (Component)
PETase site mutation S92A - F

BBa_K2110810
BBa_K2110810 Version 1 (Component)
PETase site mutation S92A - R

BBa_K2110811
BBa_K2110811 Version 1 (Component)
PETase site mutation Q119A - F

BBa_K2110812
BBa_K2110812 Version 1 (Component)
PETase site mutation Q119A - R

BBa_K2110813
BBa_K2110813 Version 1 (Component)
PETase site mutation M154L - F

BBa_K2110814
BBa_K2110814 Version 1 (Component)
PETase site mutation M154L - R

BBa_K2110815
BBa_K2110815 Version 1 (Component)
PETase site mutation M154A - F

BBa_K2110816
BBa_K2110816 Version 1 (Component)
PETase site mutation M154A - R

BBa_K2110817
BBa_K2110817 Version 1 (Component)
PETase site mutation W159H - F

BBa_K2110818
BBa_K2110818 Version 1 (Component)
PETase site mutation W159H - R

BBa_K2110819
BBa_K2110819 Version 1 (Component)
PETase site mutation W159M - F

BBa_K2110820
BBa_K2110820 Version 1 (Component)
PETase site mutation W159M - R

BBa_K2110821
BBa_K2110821 Version 1 (Component)
PETase site mutation W159A - F

BBa_K2110822
BBa_K2110822 Version 1 (Component)
PETase site mutation W159A - R

BBa_K2110823
BBa_K2110823 Version 1 (Component)
PETase site mutation N205V - F

BBa_K2110824
BBa_K2110824 Version 1 (Component)
PETase site mutation N205V - R

BBa_K2110825
BBa_K2110825 Version 1 (Component)
PETase site mutation N205L - F

BBa_K2110826
BBa_K2110826 Version 1 (Component)
PETase site mutation N205L - R

BBa_K2110827
BBa_K2110827 Version 1 (Component)
PETase site mutation S207T - F

BBa_K2110828
BBa_K2110828 Version 1 (Component)
PETase site mutation S207T - R

BBa_K2110829
BBa_K2110829 Version 1 (Component)
PETase site mutation S207A - F

BBa_K2110830
BBa_K2110830 Version 1 (Component)
PETase site mutation S207A - R

BBa_K2110831
BBa_K2110831 Version 1 (Component)
PETase site mutation I208V - F

BBa_K2110832
BBa_K2110832 Version 1 (Component)
PETase site mutation I208V - R

BBa_K2110833
BBa_K2110833 Version 1 (Component)
PETase site mutation S238F - F

BBa_K2110834
BBa_K2110834 Version 1 (Component)
PETase site mutation S238F - R

BBa_K2110835
BBa_K2110835 Version 1 (Component)
PETase site mutation S238V - F

BBa_K2110836
BBa_K2110836 Version 1 (Component)
PETase site mutation S238V - R

BBa_K2110837
BBa_K2110837 Version 1 (Component)
PETase site mutation A240P - F

BBa_K2110838
BBa_K2110838 Version 1 (Component)
PETase site mutation A240P - R

BBa_K2110839
BBa_K2110839 Version 1 (Component)
PETase site mutation S242I - F

BBa_K2110840
BBa_K2110840 Version 1 (Component)
PETase site mutation S242I - R

BBa_K2027010
BBa_K2027010 Version 1 (Component)
Repetitive Insertion of 4D Melanin-Binding Heptapeptide

BBa_K1875010
BBa_K1875010 Version 1 (Component)
Base 10 target mutation to make mutated guide operator 13

BBa_K2105000
BBa_K2105000 Version 1 (Component)
LacZ delta M15 mutation

BBa_K1987000
BBa_K1987000 Version 1 (Component)
GFP with BsaI sites for promoter insertion

dCas9 B
BBa_K1903002 Version 1 (Component)
dCas9 with protein insertion frames version B

dCas9 A
BBa_K1903001 Version 1 (Component)
dCas9 with protein insertion frames version A

BBa_K1903022
BBa_K1903022 Version 1 (Component)
Insertion 1 for dCas9 version B

BBa_K1903023
BBa_K1903023 Version 1 (Component)
Insertion 2 for dCas9 version B

Insertion
BBa_K1903020 Version 1 (Component)
Insertion 1 for dCas9 version A

Insertion
BBa_K1903021 Version 1 (Component)
Insertion 2 for dCas9 version A

BBa_K371051
BBa_K371051 Version 1 (Component)
pSB1C5 (1bp mutation of pSB1C3 to remove SacI)

BBa_M36111
BBa_M36111 Version 1 (Component)
Lysis gene E for phiX174, synonymous mutation on gene J's rbs inside E

BBa_J119009
BBa_J119009 Version 1 (Component)
pLacI-RBS-T7RNAP with sites for oligo insertion

pINDEL
BBa_K551001 Version 1 (Component)
plasmid of insertion and deletion

BBa_K764005
BBa_K764005 Version 1 (Component)
WbbC with point mutation

BBa_K764012
BBa_K764012 Version 1 (Component)
WbbC with point mutation + BBa_B0014

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o