BBa_K395604BBa_K395604 Version 1 (Component)MpAAT1 coding region
BBa_M1350BBa_M1350 Version 1 (Component)hbd coding region
BBa_M1131BBa_M1131 Version 1 (Component)5' flanking region of Lactase-phlorizin hydrolase (LPH), eukaryotic
BBa_M1351BBa_M1351 Version 1 (Component)Thiolase coding Region
BBa_K656002BBa_K656002 Version 1 (Component)Cohesin region of Scaffolding Protein A from Clostridium thermocellum
BBa_K648029BBa_K648029 Version 1 (Component)OR, operating region for Lambda switch
BBa_K558000BBa_K558000 Version 1 (Component)PDC/ADH coding regions
BBa_K558008BBa_K558008 Version 1 (Component)Bay Laurel Thioesterase coding region
OLBBa_K566000 Version 1 (Component)OL region from Lambda
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
mtrBBBa_K098989 Version 1 (Component)mtrB coding region
BBa_K098997BBa_K098997 Version 1 (Component)cIts coding region
BBa_K098998BBa_K098998 Version 1 (Component)cIts coding region with rbs
BBa_K157002BBa_K157002 Version 1 (Component)Transmembrane region of the EGF-Receptor (ErbB-1)
BBa_K157010BBa_K157010 Version 1 (Component)glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac
OriTRBBa_J01003 Version 1 (Component)OriT-R (Origin of transfer for the R-plasmid nic region)
RegionalBBa_K106676 Version 1 (Component)Regional silencing construct
KaiABBa_J36801 Version 1 (Component)KaiA coding region
KaiBBBa_J36802 Version 1 (Component)KaiB coding region
KaiCBBa_J36804 Version 1 (Component)KaiC coding region
BBa_K116601BBa_K116601 Version 1 (Component)HtlB (ftsH) coding region from E. coli
BBa_K127011BBa_K127011 Version 1 (Component)cheR coding region
BBa_K127012BBa_K127012 Version 1 (Component)cheZ coding region
CIIBBa_K116602 Version 1 (Component)CII coding region from λ phage
CIIICdBBa_K116609 Version 1 (Component)CIIICd: modified CIII coding region from λ phage
BBa_M11076BBa_M11076 Version 1 (Component)NrtA: nitrate/nitrite binding protein coding region
blr promotBBa_K238000 Version 1 (Component)YcgF/E regulated promoter region
VirBBBa_K238011 Version 1 (Component)VirB promoter region
cadA promoBBa_K285101 Version 1 (Component)cadA regulatory region
copZA promBBa_K285102 Version 1 (Component)copZA regulatory region
BBa_K285103BBa_K285103 Version 1 (Component)mntH regulatory region
BBa_K200015BBa_K200015 Version 1 (Component)Coding region for the DpnII restriction enzyme.
BBa_K182004BBa_K182004 Version 1 (Component)Lambda CI promoter (BBa_R0051) with a point mutation in -35 region.
BBa_K249029BBa_K249029 Version 1 (Component)rpsA TIR (Translation Initiation Region)
BBa_K189044BBa_K189044 Version 1 (Component)nonstructural protein coding region of AAV
BBa_K242051BBa_K242051 Version 1 (Component)Essential region of P4 bacteriophage genome
BBa_K367002BBa_K367002 Version 1 (Component)prhI coding region
PfecABBa_K367007 Version 1 (Component)PfecA promoter region
PprhJBBa_K367008 Version 1 (Component)PprhJ promoter region
PfecABBa_K367009 Version 1 (Component)PfecA promoter region
BBa_K351001BBa_K351001 Version 1 (Component)Human STAT1 coding region
BBa_J70613BBa_J70613 Version 1 (Component)RFC12 GFP coding region
BBa_J70625BBa_J70625 Version 1 (Component)RFC12 RFP Coding region