Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caroline Dahl
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:03
Cell replication --> YFP on/off
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_I742128_sequence (Version 1) |
Description
This device allows recombination at the inverted repeats of the dif-site so that the flanked sequence between (the lac promoter) is inverted and can activate the downstream reporter gene (YFP). It was planned as the proof-of-concept for Edinburgh 2007 project.Notes
Rumour (unpublished data) has it that the flanked DNA needs be of certain lenght to allow it to form a loop and subsequently invert. 200bp is minimal so the Plac was extended to include nonfunctional LacZ C-terminus.Source
Dif-sites and the Plac are innate E. coli K12 loci. See BBa_E0430 for details about YFP.| Access | Instance | Definition |
| public public public public public public public | BBa_I742101 BBa_I742124 BBa_I742102 BBa_B0034 BBa_E0030 BBa_B0010 BBa_B0012 | difF Plac(rev) difR BBa_B0034 eyfp BBa_B0010 BBa_B0012 |