Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caroline Dahl
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:03
Division-PoPper: Cell division --> PoPS
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_I742132_sequence (Version 1) |
Description
This device gives PoPS-output on cell division. The DNA flanked by the opposing repeats of the dif-site is inverted and allows transcription of both any downstream element and a repressor which, upon sufficient build-up, switches off the promoter which controls downstream transcription. Inversion only occurs when the XerCD complex is activated, and this only occurs at cell division.Therefore this device produces a short burst of PoPS as a function of cell division. Any added downstream elements will likewise be activated, briefly, as a function of cell division.
Notes
Again, please refer to to part in question. However, as a general rule, all parts in 'non-default', bottom DNA strand need be synthesised in reverse complementary fashion. You will encounter parts that are called 'upside-down' or 'reverse complementary' for this reason.Source
Please refer to individual parts.They are all more or less modified versions of E. coli K12 loci and its phage lambda.
| Access | Instance | Definition |
| public public public public public public public public public public | BBa_I14032 BBa_I742101 BBa_I742126 BBa_B0034 BBa_C0040 BBa_I742129 BBa_I742131 BBa_I742130 BBa_R0040 BBa_I742102 | P(Lac) IQ difF revPlam BBa_B0034 tetR BBa_I742129 rev.cI revRBS p(tetR) difR |