Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Wyatt
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:04
AIP generator + mCherry + term
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_I746002_sequence (Version 1) |
Description
This part is simply the AIP generator (I746000) from the agr system with an mCherry+term device (J06702) downstream.The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.
In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.
This part was built to check for transcriptional readthrough - i.e. that I746000 does not contain a cryptic terminator or some other problematic feature. It's also handy for tagging sender cells as red fluorescent (if it works!).
Notes
N/ASource
Standard assembly of I746000 with J06702.| Access | Instance | Definition |
| public public public public public | BBa_I746000 BBa_B0034 BBa_J06504 BBa_B0010 BBa_B0012 | BBa_I746000 BBa_B0034 mCherry BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I746000 BBa_B0034 BBa_J06504 BBa_B0010 BBa_B0012 | 1,759 768,779 786,1499 1508,1587 1596,1636 | BBa_I746000 BBa_B0034 mCherry BBa_B0010 BBa_B0012 |