BBa_K1212010

BBa_K1212010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1212010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amy Soon
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:41

pBAD+Riboswitch1+GFP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1212010_sequence (Version 1)

Description

Testing construct. GFP expression is controlled by arabinose and theophylline via the pBAD promoter and the theophylline riboswitch.

Notes

The end of the riboswitch sequence must be directly before the start codon of the coding sequence for GFP. There cannot be a scar.

Source

pBAD and GFP are from the iGEM distribution kit plates and the riboswitch is from the Gallivan Lab's paper Synthetic riboswitches that induce gene expression in diverse bacterial species. by Topp et al. in Appl Environ Microbiol.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1212010/1