BBa_K143074

BBa_K143074 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K143074
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:10:24

AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K143074_sequence (Version 1)

Description

AmyE 5' Integration sequence(BBa_K143001) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts BBa_K143053 and BBa_K143064) and the PoPS generator Pveg-spoVG (BBa_K143053).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence(BBa_K143002) is cloned onto the 3' end of the construct.

The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.

The Pveg-spoVG promoter and RBS for B. subtilis constitutively generate a PoPS output.

Notes

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-spoVG was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.

Source

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-spoVG that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.

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Planning
 
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BBa_K143074/1