BBa_K1459017

BBa_K1459017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1459017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grzegorz Ścibisz

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:32

pmrC-GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1459017_sequence (Version 1)

Description

This is pmrC promoter from Salmonella enterica, with subsequent GFP. This part is one part of PmrA-PmrB detecting system. Upon phosphorylation by PmrB, PmrA binds to pmrC and induces expression of GFP. PmrB gains kinase activities after binding ions by it's extracellular loop (eg. iron or terbium atoms, depending on present binding tag).

Notes

Apart from amplifying the sequence of interest we had to also perform a directed mutagenesis to eradicate XbaI site from pmrC sequence.

Source

We obtained pmrC-GFP sequence thanks to kindness of prof. He from the University of Chicago.

Components

Access	Instance	Definition
public	BBa_K1459006	BBa_K1459006
public	BBa_E0040	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1459006	1,46	BBa_K1459006
BBa_E0040	53,772	GFP

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1459017/1

Member of these Collections

• iGEM Parts Registry